ExFLP-23 Determination of Extraneous Material in Spirulina, Kelp and Sea Vegetation
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760D92A5257749749EC788D2F553471F |
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文件大小(MB)： |
0.02 |
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页数： |
5 |
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日期： |
2012-3-3 |
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Published by: POLYSCIENCE PUBLICATIONS, P.O. Box 1606, Station St-Martin, Laval, Quebec,Canada H7V 3P9. TEL.: 1-800-840-5870. FAX: (450) 688-1930.,Government of Canada Gouvernement du Canada,Laboratory Procedure ExFLP-23,April 1997,HEALTH PROTECTION BRANCH,OTTAWA,DETERMINATION OF EXTRANEOUS MATERIAL IN SPIRULINA, KELP AND SEA VEGETATION,M.-A. Rivers,Research Division, Bureau of Microbial Hazards,Food Directorate, Postal Locator:2204A2,Health Canada, Ottawa, Ontario, K1A 0L2,1. APPLICATION,This method is applicable to the sampling and examination of spirulina, kelp and sea vegetation for extraneous,material, such as insects, rodent hairs, and feather barbules to determine compliance with Sections 4, 5 and 7,of the Food and Drugs Act. This method replaces ExFLP-23 dated September 1987.,2. DEFINITION OF TERMS,A lot is defined as that amount (volume, weight, etc.) of the food which is produced, stored and/or shipped under,conditions as nearly uniform as possible, preferably designated by a common container code or marking, and,in any event, consisting of not more than one variety, grade or type of product from a single identifiable source.,3. COLLECTION OF SAMPLES,3.1 Scrutinize the entire lot for live infestation. If live infestation is found, do not sample until after fumigation,or other effective treatment.,3.2 Obtain three sample units selected at random from the lot of at least 25 g each using appropriate,sampling equipment and containers. Three sample units constitute a sample.,3.3 Each sample unit must be kept separate and labelled 1, 2, 3. Complete information respecting the lot,size, weight of individual containers, country of origin, exporter, importer or domestic manufacturer and,product and lot identification should be recorded and should accompany the sample.,4. MATERIALS AND SPECIAL EQUIPMENT,1) Balance,2) 2-2.0 L beakers and lids (watch glasses),3) Smooth magnetic stirring bar, teflon coated (1x5 cm),4) Stirrer hot plate,5) U.S. standard No. 230 sieve,6) Shallow pan > 20 cm in diameter,ExFLP-23,- 2 - April 1997,7) 2 L percolator, premarked at 250 and 1700 mL levels,8) Wash bottles,9) Suction filtration apparatus with Buchner or Hirsch funnel (5-7 cm perforated plate),10) Ruled filter paper. Filter paper should be larger than funnel plate (7-9 cm),11) Petri dishes to suit size of filter paper used,12) Rubber policeman attached to a long glass rod,13) Stereoscopic microscope (10-30x),14) Ashless filter paper. Filter paper should be larger than funnel plate (7-9 cm),15) Drying oven (40-60oC),16) Crucible,17) Desiccator,18) Muffle oven,19) Concentrated HCl,20) Alcohol (95% ethanol),21) Mineral oil. Paraffin oil, white, light 125/135 Saybolt Universal Viscosity (38oC), specific gravity,0.840-0.860 (24oC),22) Detergent (1% sodium lauryl sulphate),23) Glycerol-alcohol (95%) mixture (1:1),24) Chloroform (CHCl3),5. PROCEDURE,The examination shall be carried out in accordance with the following instructions.,5.1 Preparation of Analytical Units,5.1.1 Thoroughly mix a sample unit and in a random fashion weigh 25 g. This 25 g constitutes an analytical,unit.,5.1.2 Repeat Step 5.1.1 for each of the remaining two sample units.,5.2 Isolation - Light Filth - Spirulina,5.2.1 Transfer an analytical unit into a 2 L beaker.,5.2.2 Add 600 mL of water and a magnetic stirring bar. Dissolve the tablets by stirring them with gentle heat,on a stirrer hot plate (0-2 hrs). Add 18 mL of HCl and digest by boiling mixture at medium heat for 30,min on a stirrer hot plate with constant magnetic stirring.,ExFLP-23,- 3 - April 1997,5.2.3 Transfer contents of beaker to a No. 230 sieve. Wash contents of sieve with a stream of hot (50-70oC),tap water until wash water runs clear and the water runs freely through the material. Defat as follows:,Place sieve in a shallow pan, cover residue to a depth of approximately 2 cm with ethanol, leave for 5,min and drain. Repeat defatting procedure once. Wet sieve spirulina until rinse water is clear. Transfer,sieve retainings to original beaker using hot (50-70oC) water to assist in transfer and dilute to,approximately 1 L.,5.2.4 Add a magnetic stirring bar, 50 mL of mineral oil and place on a heated hot plate.,5.2.5 Stir magnetically at maximum speed that does not produce visible or audible splashing for 5 min.,5.2.6 Promptly transfer contents of beaker to a percolator containing approximately 250 mL of water. Rinse,contents of beaker into percolator with hot tap water making certain to transfer all material including,heavy filth. Bring volume in percolator to 1700 mL with hot tap water.,5.2.7 Using wash bottles, rinse magnetic particles from stirring bar into a b……
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